agarose gel คือ: นี่คือโพสต์ที่เกี่ยวข้องกับหัวข้อนี้
Three makes a happy family
The core Midori Green molecule has been formulated into three different stains optimized for your lab’s needs. First, there is Midori Green Advance – which offers an excellent signal-to-noise ratio and good sensitivity for even short nucleic fragments. Midori Green Advance is added into the agarose gel prior to running samples (much like ethidium bromide) and provides sensitivity of detection on par with EtBr when visualized with blue LEDs, or Nippon Genetics’ novel blue/green LED technology. The second dye, Midori Green Direct, is designed to simply mix with your sample and load onto a stain-free gel. Midori Green Direct has the loading dye included and detects nucleic acid fragments to a similar, if not slightly better, level as Midori Green Advanced. The newest member of the family is Midori Green Xtra. Like “Advance”, Midori Green Xtra is added to the gel and buffer. It’s chemical structure is optimized for Blue/Green and Blue LED light, leading to unbeatable fluorescence signals of DNA and RNA in agarose gels. All three dyes are compatible with UV-light, but are slightly less efficient when using UV rather than the non-damaging visible excitation light of Blue and Blue/Green LEDs. Most important for labs, Midori Green Xtra does not stain the agarose gel, leading to an excellent signal-to-noise ratio, making the detection of even the minutest quantities of DNA or RNA possible ( and certainly better than EtBr).
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[Update] Gelation mechanism of agarose ☆ | agarose gel คือ – Australia.xemloibaihat
The non-Newtonian behavior and dynamic viscoelasticity of a series of aqueous solutions of agarose were measured with a rheogoniometer. The flow curve, at 25°, of agarose solution approximated to plastic behavior at 0.1, 0.13, and 0.15% concentrations. Gelation occurred at concentration of 0.13% at low temperature (0°). The dynamic modulus of agarose showed a very high value at low temperature, and increased with an increase in temperature, showing a maximum value at 30°, then it decreased. In the presence of NaCl, KCl, CaCl2, and MgCl2 for a solution of agarose at 0.08% concentration, the transition temperature, at which dynamic modulus decreased rapidly, was observed at 60°. Gelation was also observed at low temperature (0°) in acid and alkaline range after reaching pH values of 2.3 and 9.5, respectively, by addition of 100mm HCl, H2SO4, NaOH, and Ca(OH)2 to a 0.08% agarose solution. A possible mode of intra- and inter-molecular hydrogen bonding within and between the agarose molecules in aqueous solution is proposed.
What Is Capillary Electrophoresis?
Have you ever wondered how capillary electrophoresis works? Watch this animation to learn the basics of this powerful technology for separating DNA! For additional resources, please visit https://bit.ly/2L9gkQB
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Polyacrylamide Gel Electrophoresis- PAGE – Amrita University
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PAGE (Polyacrylamide Gel Electrophoresis), is the most widely used analytical method to resolve separate components of a protein mixture based on size.
For this protein molecules of different shapes and sizes, need to be denatured. This is done with the aid of SDS, so that the proteins no longer have any secondary, tertiary or quaternary structure. The proteins covered by SDS are negatively charged. When loaded onto a gel matrix and placed in an electric field, the proteins will migrate towards the anode (positively charged electrode). They are then separated by a molecular sieving effect based on size. After visualization by a proteinspecific staining technique, the size of a protein can be estimated by comparison of its migration distance with that of a known molecular weight marker.
What is Gel Electrophoresis | Don’t Memorise
How do we separate the desired DNA fragment from a complete mixture of several DNA fragments? The job sounds almost impossible! But there is a technique that comes to our rescue! Gel Electrophoresis is a separation technique which separates DNA fragments based on their Length or Size. Want to know how the technique works? Watch this video to understand the Principle and Technique of Gel Electrophoresis.
In this video, we will learn:
0:00 Introduction
0:44 definition of Gel Electrophoresis
1:19 is DNA charged?
2:20 principle and working of Gel Electrophoresis
2:48 principle of separation of the DNA fragments
3:57 requirements for Gel Electrophoresis
3:55 Gel Electrophoresis process
6:24 DNA separation
7:25 Elution
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GelElectrophoresis AgaroseGel Electrophoresis
How to Make an SDS-PAGE gel
Alright so here’s a quick video on how to cast an SDSPAGE gel. Although recipes can vary, the ingredients shown here are almost always used. Remember: always add TEMED last, and pour into plates immediately after!\r
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Already know how to make a gel, but have problems with leaky gels? Check out our other video on How to Avoid a Leaky SDSPAGE gel: http://www.youtube.com/watch?v=b45nSOyPP_4\r
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Running an Agarose Gel – University of Leicester
A short film showing the procedures involved in the production and running of an agarose gel.\r
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The film was produced by GENIE, based within the Genetics Department at the University of Leicester. Further information and materials can be found on the GENIE web site http://www.le.ac.uk/ge/genie/ and the GENIE VGEC web site http://www.le.ac.uk/ge/genie/vgec/\r
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The film was directed by Jon Shears of ITSMultimedia Services and the film was produced by Raymond Dagleish, Nicola SuterGiorgini and Cas Kramer.
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